An Interactive Annotated World Bibliography of Printed and Digital Works in the History of Medicine and the Life Sciences from Circa 2000 BCE to 2022 by Fielding H. Garrison (1870-1935), Leslie T. Morton (1907-2004), and Jeremy M. Norman (1945- ) Traditionally Known as “Garrison-Morton”
Permanent Link for Entry #16285
Architecture of RNA polymerase II and implications for the transcription mechanism.Science, 288, 640-649, 2000.
Kornberg (Nobel Prize 2007) devoted two decades to the development of methods to visualize the atomic structure of RNA polymerase and its associated protein components. Initially, Kornberg took advantage of expertise with lipid membranes gained from his graduate studies to devise a technique for the formation of two-dimensional protein crystals on lipid bilayers. These 2D crystals could then be analyzed using electron microscopy to derive low-resolution images of the protein's structure. Eventually, Kornberg was able to use X-ray crystallography to solve the 3-dimensional structure of RNA polymerase at atomic resolution. Through these studies, Kornberg created an actual picture of how transcription works at a molecular level. According to the Nobel Prize committee, "the truly revolutionary aspect of the picture Kornberg has created is that it captures the process of transcription in full flow. What we see is an RNA-strand being constructed, and hence the exact positions of the DNA, polymerase and RNA during this process.” With P Cramer , D A Bushnell, J Fu, A L Gnatt, B Maier-Davis, N E Thompson, R R Burgess, A M Edwards, P R David. Digital facsimile from PubMedCentral at this link.
Subjects: BIOLOGY › MOLECULAR BIOLOGY › Protein Structure, BIOLOGY › MOLECULAR BIOLOGY › Protein Synthesis