An Interactive Annotated World Bibliography of Printed and Digital Works in the History of Medicine and the Life Sciences from Circa 2000 BCE to 2022 by Fielding H. Garrison (1870-1935), Leslie T. Morton (1907-2004), and Jeremy M. Norman (1945- ) Traditionally Known as “Garrison-Morton”

15961 entries, 13944 authors and 1935 subjects. Updated: March 22, 2024


1 entries
  • 13980

Expression in Escherichia coli of chemically synthesized genes for human insulin (plasmid construction / lac operon / fused proteins / radioimmunoassay / peptide purification).

Proc. Nat. Acad. Sci. (USA), 76, 106-110, 1979.

Working at Genentech, Kleid and Goeddel and colleagues were the first scientists to apply genetic engineering techniques, incorporating chemically synthesized DNA encoding human insulin into E. coli, that expressed, after appropriate treatment, synethetic human insulin. Digital facsimile from PubMedCentral at this link.

Subjects: BIOLOGY › MOLECULAR BIOLOGY › Recombinant DNA, Biotechnology, Metabolism & Metabolic Disorders › Diabetes