CHALFIE, Martin Lee
Chalfie (Nobel Prize 2008) and colleagues showed that the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, could be used as a visible marker for protein localization and expression in vivo, in bacteria and worm cells, and in the absence of any contributing factors from the jelly fish itself, proving that the protein acted totally alone without any interference from possible contaminating factors. This demonstration established GFP as a fine tool to study proteins in vivo, and fundamentally altered the way in which investigators could define and study intracellular and intra organismal biological events. Douglas Prasher played a key role in this discovery, but did not share in the Nobel Prize.
Subjects: BIOCHEMISTRY › Bioluminescence, BIOLOGY › MOLECULAR BIOLOGY › Protein Structure, BIOLOGY › MOLECULAR BIOLOGY › Protein Synthesis