Mechanism of DNA chain growth, I. Possible discontinuity and unusual secondary structure of newly synthesized chains.Proc. Nat. Acad. Sci. (USA), 59, 598-605, 1968.
The Okazakis discovered what became known as Okazaki fragments, short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA to create the lagging strand during DNA replication. Before this discovery it was commonly thought that replication was a continuous process for both strands, but the discoveries involving E. coli led to a new model of replication. The Ozakis found that there was a discontinuous replication process by pulse-labeling DNA and observing changes that pointed to non-contiguous replication.
Subjects: BIOLOGY › MOLECULAR BIOLOGY › Nucleic Acids